Journal: Journal of Nanobiotechnology

Article Title: Microneedles combining delivery of hUMSC-derived exosomes and EGCG mitigate UV-induced skin damage

doi: 10.1186/s12951-025-03735-x

Figure Lengend Snippet: hUMSC-Exo extraction and characterization. (A) FCM detection of markers of hUMSCs, which express CD29 and CD90 and do not express hematopoietic lineage markers CD34 and CD45. (B) Schematic representation of the hUMSC-Exo extraction process. (C) Particle size distribution analysis of hUMSC-Exo. (D) Representative images of TEM scans depicting the morphology of hUMSC-Exo. (E) Western blot analysis of protein expression profiles in hUMSCs and hUMSC-Exo. (F) Representative immunofluorescence images showing HaCaT cells internalizing hUMSC-Exo. Blue: nucleus (Hoechst 33258), green: cytoskeleton (phalloidin), and red: hUMSC-Exo (PKH26)

Article Snippet: An IL-1β activator (20 ng·mL−1; IL-1 beta protein, Human, HY-P7028, MCE) and an SOD2 inhibitor (10 μM; 2-Methoxyestradiol, 2-ME, HY-12033, MCE) were applied to HaCaT cells in the presence of Exo/EGCG for 24 h prior to UVB exposure.

Techniques: Extraction, Western Blot, Expressing, Immunofluorescence

Journal: Journal of Nanobiotechnology

Article Title: Microneedles combining delivery of hUMSC-derived exosomes and EGCG mitigate UV-induced skin damage

doi: 10.1186/s12951-025-03735-x

Figure Lengend Snippet: hUMSC-Exo/EGCG inhibits UV-induced oxidative damage, DNA damage and anti-inflammatory activity in HaCaT. (A) Pro-cell proliferative capacity of hUMSC-Exo, EGCG and hUMSC-Exo/EGCG. (B) Representative images of the effects of hUMSC-Exo, EGCG and hUMSC-Exo/EGCG on ROS fluorescence levels in oxidatively damaged HaCaT. (C) Effect of hUMSC-Exo, EGCG and hUMSC-Exo/EGCG on the activity of oxidatively damaged HaCaT by CCK-8 assay. (D) Flow cytometry detection of hUMSC-Exo, EGCG and hUMSC-Exo/EGCG on ROS in oxidatively damaged HaCaT by fluorescence intensity and statistical analysis. (E) DPPH assay for clear anti-ROS ability of hUMSC-Exo/EGCG. (F) Representative confocal microscopy images of the effects of hUMSC-Exo, EGCG and hUMSC-Exo/EGCG on the fluorescence level of γ-H2AX in oxidatively damaged HaCaT. Blue: nucleus, Red: γ-H2AX. The secretion of (G) TNF-α, (H) IL-6 and (I) IL-1β in supernatants of HaCaT cells after indicated treatments was detected by ELISA kit. n ≥ 3. Significances are presented by * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: An IL-1β activator (20 ng·mL−1; IL-1 beta protein, Human, HY-P7028, MCE) and an SOD2 inhibitor (10 μM; 2-Methoxyestradiol, 2-ME, HY-12033, MCE) were applied to HaCaT cells in the presence of Exo/EGCG for 24 h prior to UVB exposure.

Techniques: Activity Assay, Fluorescence, CCK-8 Assay, Flow Cytometry, DPPH Assay, Confocal Microscopy, Enzyme-linked Immunosorbent Assay

Journal: Journal of Nanobiotechnology

Article Title: Microneedles combining delivery of hUMSC-derived exosomes and EGCG mitigate UV-induced skin damage

doi: 10.1186/s12951-025-03735-x

Figure Lengend Snippet: hUMSC-Exo/EGCG alleviates UVB-induced photodamage by suppressing IL-1β and activating SOD2. (A) mRNA expression levels of differentially expressed genes (DEGs) in HaCaT cells from different treatment groups, as determined by qRT-PCR. (B) Protein expression levels of DEGs in UVB-irradiated HaCaT cells, analyzed by Western blot. (C) Secretion levels of IL-6, and IL-1β in supernatants of HaCaT cells were measured using ELISA kits. (D) Representative fluorescence images showing reactive oxygen species (ROS) levels and DNA damage in HaCaT cells. Green: DCFH-DA (ROS probe); Blue: Nuclei (Hoechst); Red: γ-H2AX (DNA damage marker). (E) Flow cytometric quantification and statistical analysis of ROS levels in oxidatively damaged HaCaT cells following IL-1β activation or SOD2 inhibition. Data are representative of at least three independent experiments. n ≥ 3. Significance is indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: An IL-1β activator (20 ng·mL−1; IL-1 beta protein, Human, HY-P7028, MCE) and an SOD2 inhibitor (10 μM; 2-Methoxyestradiol, 2-ME, HY-12033, MCE) were applied to HaCaT cells in the presence of Exo/EGCG for 24 h prior to UVB exposure.

Techniques: Expressing, Quantitative RT-PCR, Irradiation, Western Blot, Enzyme-linked Immunosorbent Assay, Fluorescence, Marker, Activation Assay, Inhibition